RANIFEN CT a brand of Trypsin and chymotrypsin are both serine proteases with high sequence and structural similarities, but with different substrate specificity. Previous experiments have demonstrated the critical role of the two loops outside the binding pocket in controlling the specificity of the two enzymes. To understand the mechanism of such a control of specificity by distant loops.

We have used the Gaussian network model to study the dynamic properties of RANIFEN CT TAB (trypsin and chymotrypsin) and the roles played by the two loops. A clustering method was introduced to analyze the correlated motions of residues. We have found that RANIFEN CT TAB of trypsin and chymotrypsin have distinct dynamic signatures in the two loop regions, which are in turn highly correlated with motions of certain residues in the binding pockets. Interestingly, replacing the two loops of RANIFEN CT TAB trypsin with those of chymotrypsin changes the motion style of trypsin to chymotrypsin-like, whereas the same experimental replacement was shown necessary to make trypsin have chymotrypsin’s enzyme specificity and activity. These results suggest that the cooperative motions of the two loops and the substrate-binding sites contribute to the activity and substrate specificity of trypsin and chymotrypsin.

RANIFEN CT TAB Trypsin , chymotrypsin , Aceclofena with Paracetamol is an oral proteolytic enzyme preparation which has been in clinical use since the 1960s. It provides better resolution of inflammatory symptoms and promotes speedier recovery of acute tissue injury than several of the other existing enzyme preparations.

RANIFEN CT combination of Trypsin and chymotrypsin along with (Aceclofena Paracetamol) are important digestive enzymes that are secreted by the pancreas as the inactive enzyme precursors trypsinogen and chymotrypsinogen. Trypsin activates itself via positive feedback and converts chymotrypsinogen and other inactive enzymes into their active forms. As these enzymes contain serine as the functional amino acid in the active centre, they are classified within the group of serine endopeptidases. Trypsin and chymotrypsin possess a very similar tertiary structure, although they cleave proteins at different positions in the polypeptide chain as a result of different substrate binding sites.

RANIFEN CT have been used to facilitate tissue repair ever since ancient times. Trypsin: Chymotrypsin, proteolytic enzyme oral preparation which has been in clinical use ever since 1960s, provides a better resolution of inflammatory symptoms and promotes speedier recovery of acute tissue injury by minimising the fibrinolytic shut down. This paper revisits the role and clinical utility of chymotrypsin: trypsin oral combination in tissue repair, in surgical patients. The aim of the present study was to determine the efficacy of RANIFEN CT chymotrypsin n trypsin in reducing post-operative oedema and haematoma formation and its role in surgical prophylaxis and scar formation.

Methods: A prospective study was done on 50 electively posted surgical patients during a period of 6months comparing placebo with chymotrypsin and trypsin oral combination.

Results: Statistically significant reduction in post-operative oedema, seroma and haematoma formation and reduction in pain and incidence of suture site infection, better cosmetic appearance of the scar and decline in need for secondary suturing was noted.